Opentrons Integration

Overview

Create production-minded Python Protocol API v2 protocols for Opentrons Flex and OT-2. This skill covers protocol structure, hardware and deck configuration, liquid handling, runtime customization, module control, simulation, and safe deployment.

The verified baseline as of 2026-07-23 is:

• opentrons==9.1.1 for reproducible Flex simulation.
• opentrons==9.0.0 for local OT-2 API 2.28 compatibility simulation.
• Flex supports API levels 2.15 through 2.29 on current software.
• OT-2 supports API levels 2.0 through 2.28 on current software.
• API 2.29 is Flex-only at this baseline. Do not put 2.29 in an OT-2 protocol.

Read references/sources.md for the upstream documentation used for this snapshot. Recheck the official versioning page before targeting newer robot software.

Safety Boundary

Opentrons protocols control physical equipment. Never treat successful Python syntax or local simulation as permission to run on a robot.

Before live execution:

1. Simulate locally with the same pinned opentrons version used for authoring.
2. Import the protocol into the correct Opentrons App and require successful analysis.
3. Verify ro…